human b cell lymphoma cell lines ramos  (ATCC)

Journal: Frontiers in Immunology

Article Title: Glycoengineering CAR-T cells to overcome galectin-3-mediated immunosuppression

doi: 10.3389/fimmu.2026.1766555

Figure Lengend Snippet: ST6 OE CAR-T cells exhibited effective tumoricidal activity in the presence of Gal-3 and evaded Gal-3-induced expression of IL-5, which compromised anti-tumor efficacy. Day 10-expanded T cells, CAR-T cells, and ST6 OE CAR-T cells were incubated with Gal-3 low Raji-luc + cells or Gal-3 high SUDHL-6-luc + cells at effector:target (E:T) ratios of 20:1. Raji cell cytotoxicity was evaluated (a) without rhGal-3 or (b) with rhGal-3, and (c) Gal-3 high SUDHL-6 cell cytotoxicity was examined without exogenous rhGal-3. Data are presented from n = 4 independent donors and analyzed using a two-way repeated-measures ANOVA with Sidak’s multiple-comparisons test, with ** p < 0.01, * p < 0.05. (d) Human cytokine proteome profiling of supernatants from Day 10-expanded T cells, CAR-T cells, and ST6 OE CAR-T cells incubated with rhGal-3 for 16h revealed a marked upregulation of IL-5 (red hatched box) in CAR-T cells that was absent in ST6 OE CAR-T cells. (e) Arrays were repeated 4 times and graphed as Relative Optical Density (Mean ± SEM). Intracellular IL-5 expression was assessed by flow cytometry in control Day 10-expanded T cells, CAR-T cells and ST6 OE CAR-T cells after rhGal-3 incubation for 16h and mean ± SEM fold change from n = 4 independent donors was graphed (f) (* p < 0.05). Statistical significance was determined using a Mann–Whitney U test. CAR-T cells and ST6 OE CAR-T cells were incubated with (g) Gal-3 low Raji-luc + cells or (h) Gal-3 high SUDHL6-luc + at E:T ratios of 20:1 (** p <0.01, *** p <0.001). Statistical significance was determined using two-way repeated-measures ANOVA with Sidak’s multiple-comparisons test.

Article Snippet: Human B-lymphoma Raji cell line expressing luciferase (Raji-Luc + ) purchased from the ATCC (Manassas, VA).

Techniques: Activity Assay, Expressing, Incubation, Flow Cytometry, Control, MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Glycoengineering CAR-T cells to overcome galectin-3-mediated immunosuppression

doi: 10.3389/fimmu.2026.1766555

Figure Lengend Snippet: ST6 OE CAR-T cells exhibited potent in vivo anti-tumor activity and persisted longer in vivo than conventional CAR-T cells. Using a DLBCL xenograft model (a) , NSG mice were inoculated with Raji-luc + cells and, after 7 days, treated with media (control), Day 10-expanded T cells, CAR-T cells, or ST6 OE CAR-T cells. Tumor growth in NSG mice was monitored weekly by in vivo BL imaging system (b) , and BLI measurements reflecting tumor burden from respective groups were graphed as shown (n = 9 mice per group) (** p < 0.01) (c) . Xenografted mouse survival data were graphed as shown (Gehan-Breslow-Wilcoxon test – ** p = 0.0106) (d) , and flow cytometry of CAR + T cells isolated from xenografted mouse spleens expressed as Mean ± SEM were graphed as shown (Mann–Whitney U test- * p <0.05) (e) .

Article Snippet: Human B-lymphoma Raji cell line expressing luciferase (Raji-Luc + ) purchased from the ATCC (Manassas, VA).

Techniques: In Vivo, Activity Assay, Control, Imaging, Flow Cytometry, Isolation, MANN-WHITNEY

Journal: Materials Today Bio

Article Title: Tumor-homing exosomes enable targeted delivery of siRNA and isoimperatorin for overcoming BTK inhibitor resistance in DLBCL

doi: 10.1016/j.mtbio.2025.102267

Figure Lengend Snippet: Roles of ISOIM/siBTK@Exosome on T cells activation and macrophage polarization. A, Experimental scheme for the co-culture of stimulated CD3 + T cells and treated SU-DHL-4 cells; B-C, Representative images (B) and corresponding quantitative analysis (C) showing the percentages of CD69 + CD4 + T cells and CD69 + CD8 + T cells in the coculture system; D, Experimental scheme for the co-culture of stimulated peritoneal macrophages and treated SU-DHL-4 cells; E-F, Representative images (E) and corresponding quantitative analysis (F) showing the percentages of M2 macrophages in the coculture system. ∗P < 0.05; ∗∗P < 0.01.

Article Snippet: Human B Lymphoma cell line SU-DHL-4 (#CRL-2957, ATCC, USA) and OCL-Ly8 (#JLC- G14773 , Gelatins biological reagent Co., LTD, Jiangxi, China) were cultured in the RPMI-1640 medium with an additional adding of 10 % FBS, penicillin (100 U/mL) and streptomycin (100 μg/mL) in an incubator of 5 % CO 2 at 37 °C.

Techniques: Activation Assay, Co-Culture Assay